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  • Title: Determination of riboflavin by high-performance liquid chromatography with riboflavin-depleted urine as calibration and control matrix.
    Author: Chen M, Andrenyak DM, Moody DE, Foltz RL.
    Journal: J Chromatogr B Analyt Technol Biomed Life Sci; 2005 Jun 05; 820(1):147-50. PubMed ID: 15866504.
    Abstract:
    A simple method, exposure to natural-light, was developed to remove riboflavin from urine to enhance its use as the biological matrix for the preparation of calibration and control samples. Riboflavin-depleted urine containing less than 1 ng/ml of riboflavin was used to validate a high-performance liquid chromatography with fluorescence detection method for the determination of urinary riboflavin. The linearity of the assay (r2=0.999) was acceptable over the range of 10-5000 ng/ml. The intra-assay and inter-assay CVs were 3.3% and 9%, respectively. Subsequent stability studies found that urine riboflavin was stable for up to 6 months at 4 or -20 degrees C.
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