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  • Title: Birth of a healthy baby after transfer of blastocysts derived from cryopreserved human oocytes fertilized with frozen spermatozoa.
    Author: Tjer GC, Chiu TT, Cheung LP, Lok IH, Haines CJ.
    Journal: Fertil Steril; 2005 May; 83(5):1547-9. PubMed ID: 15866601.
    Abstract:
    OBJECTIVE: To report the birth of a healthy baby after transfer of blastocysts derived from frozen eggs and frozen spermatozoa. DESIGN: Case report. SETTING: University-based assisted reproduction center. PATIENT(S): A 37-year-old woman with secondary infertility of 4 years' duration. INTERVENTION(S): Retrieved oocytes were cryopreserved in 1.5 M 1,2-propanediol (PROH) and 0.3 M sucrose by a slow freezing-rapid thawing protocol, semen cryopreservation, and insemination by intracytoplasmic sperm injection (ICSI) at 4 hours after thawing. MAIN OUTCOME MEASURE(S): Fertilization and embryo development to blastocyst stage, pregnancy, and outcome. RESULT(S): Ten of 14 frozen oocytes survived after thawing. Eight of them were fertilized by performing ICSI and three developed into 7- to 8-cell embryos on day 3. Two of these embryos developed into blastocysts on day 5 and were transferred. This resulted in a successful pregnancy and the delivery of a healthy baby boy. CONCLUSION(S): This case demonstrates the feasibility of inseminating the frozen-thawed human oocytes after 4 hours of in vitro culture and the zygotes derived from frozen oocytes and frozen spermatozoa can be cultured to blastocysts resulting in the live birth of a healthy baby boy.
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