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  • Title: Monosaccharide composition analysis of glycoproteins by isotope-tag method and capillary LC/ESI-MS.
    Author: Yuan J, Kawasaki N, Hashii N, Itoh S, Kawanishi T, Hayakawa T.
    Journal: Yao Xue Xue Bao; 2005 Jan; 40(1):43-8. PubMed ID: 15881326.
    Abstract:
    AIM: To develop a rapid and sensitive method for monosaccharide composition analysis. METHODS: Glycoprotein was first hydrolyzed to monosaccharides, which were subsequently reacetylated (amino monosaccharides), tagged with 2-aminopyridine and then separated and monitored in selected ion mode by CapGCC-LC/MS. The use of tetradeuterium labeled-aminopyridyl-monosaccharides prepared by tagging monosacahrides with hexadeuterium labeled 2-aminopyridine as internal standards improved the linearity and reproducibility in quantification. RESULTS: This method was successfully applied to monosaccharide composition analysis of model glycoproteins, fetuin and erythropoietin down to 1 pmol monosaccharides. CONCLUSION: This method has been shown to be highly sensitive and is applicable to monosaccharide composition analysis of glycoproteins.
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