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Title: Techniques for antifungal susceptibility testing of Candida albicans biofilms. Author: Ramage G, López-Ribot JL. Journal: Methods Mol Med; 2005; 118():71-9. PubMed ID: 15888936. Abstract: Candida albicans is capable of forming biofilms on a variety of inert and biological surfaces. Cells in biofilms display phenotypic properties that are radically different from their free-floating planktonic counterparts, including their recalcitrance to antimicrobial agents. Consequently, Candida biofilm-associated infections are difficult to treat and to fully eradicate with standard antifungal therapy. Here, we describe a simple, fast, inexpensive and highly reproducible microtiter plate-based assay for the antifungal susceptibility testing of C. albicans biofilms. Because of its simplicity, compatibility with widely available 96-well microplate platform, high throughput, and automation potential, this assay represents an important tool towards the standardization of in vitro antifungal susceptibility testing of fungal biofilms.[Abstract] [Full Text] [Related] [New Search]