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  • Title: [A method for the preparation of normalized cDNA libraries enriched with full-length sequences].
    Author: Zhulidov PA, Bogdanova EA, Shcheglov AS, Shagina IA, Vagner LL, Khazpekov GL, Kozhemiako VV, Luk'ianov SA, Shagin DA.
    Journal: Bioorg Khim; 2005; 31(2):186-94. PubMed ID: 15889793.
    Abstract:
    We developed a new method for the preparation of normalized cDNA libraries enriched with full-length sequences. It is based on the properties of the recently characterized duplex-specific nuclease from the hepatopancreas of the Kamchatka crab. The duplex-specific nuclease is thermostable, it effectively cleaves double-stranded DNA and is inactive toward single-stranded DNA (Shagin et al., Genome Res., 2002, vol. 12, pp. 1935-1942). Our method enables the normalization of cDNA samples enriched with full-length sequences without use of laborious and ineffective stages of physical separation. The efficiency of the method was demonstrated in model experiments using cDNA samples from several human tissues.
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