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  • Title: Cloning and characterization of ecdysone receptor and ultraspiracle cDNAs from Spodoptera litura.
    Author: Nagata S, Maruyama T, Ohira T, Wataru S, Nagasawa H.
    Journal: Ann N Y Acad Sci; 2005 Apr; 1040():417-9. PubMed ID: 15891077.
    Abstract:
    The insect molting hormone, ecdysone, induces a series of events critical for ecdysis, beginning with the formation of a complex between ecdysteroid receptor (EcR) and its partner protein, ultraspiracle (Usp). In the present study, we cloned cDNAs encoding EcR and Usp by RT-PCR and 5'-/3'-RACE from the common cutworm, Spodoptera litura, a destructive agricultural pest insect in East Asia. Hexahistidine-tagged recombinant EcR and Usp of S. litura were expressed in E. coli, and the proteins, after refolding, exhibited the specific binding to an ecdysone analogue in vitro.
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