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  • Title: Quantitating adenylate nucleotides in diverse organisms.
    Author: Napolitano MJ, Shain DH.
    Journal: J Biochem Biophys Methods; 2005 Apr 29; 63(1):69-77. PubMed ID: 15892979.
    Abstract:
    Quantitation of cellular adenylate levels (i.e., ATP, ADP, AMP) has widespread applications in physiological, metabolic and energetic studies. We have compared classical adenylate extraction procedures (i.e., perchloric acid, boiling) with a previously unreported proteinase K-based extraction technique. Our results suggest that all three techniques are comparable in soft animal tissue, but proteinase K-based extractions consistently generated higher adenylate yields from a broad range of organisms, particularly those containing a cell wall (e.g., alga, bacteria, fungi, plant).
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