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  • Title: Landscape phage probes for Salmonella typhimurium.
    Author: Sorokulova IB, Olsen EV, Chen IH, Fiebor B, Barbaree JM, Vodyanoy VJ, Chin BA, Petrenko VA.
    Journal: J Microbiol Methods; 2005 Oct; 63(1):55-72. PubMed ID: 15893394.
    Abstract:
    We selected from landscape phage library probes that bind preferentially Salmonella typhimurium cells compared with other Enterobacteriaceae. The specificity of the phage probes for S. typhimurium was analyzed by the phage-capture test, the enzyme-linked immunosorbent assay (ELISA), and the precipitation test. Interaction of representative probes with S. typhimurium was characterized by fluorescence-activated cell sorting (FACS), and fluorescent, optical and electron microscopy. The results show that the landscape phage library is a rich source of specific and robust probes for S. typhimurium suitable for long-term use in continuous monitoring devices and biosorbents.
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