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  • Title: Spectrophotometric determination of DNA using a near infrared probe 1,1'-disulfobutyl-3,3,3',3'-tetramethylindotricarbocyanine.
    Author: Wang H, Li WR, Lu Y, Fu NN, Zhang HS.
    Journal: Spectrochim Acta A Mol Biomol Spectrosc; 2005 Jul; 61(9):2103-7. PubMed ID: 15911398.
    Abstract:
    A direct spectrophotometric determination of DNA in solution has been developed using a near infrared probe, 1,1'-disulfobutyl-3,3,3',3'-tetramethylindotricarbocyanine (DSTCY). In pH = 7.5 Tris-HCl buffer, the reaction of DSTCY with DNA was complete within 10 min at room temperature in the presence of cetyltrimethylammonium bromide (CTAB), which leaded to a sharp increase of the absorbance at 474 nm. The concentration of DNA can be determined in such wavelength with the linear range of 0.5-8.0 microg ml(-1) and the detection limit of 45 ng ml(-1).
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