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  • Title: Comparative study of four extraction methods for enterovirus recovery from wastewater and sewage sludge.
    Author: Belguith K, Hassen A, Aouni M.
    Journal: Bioresour Technol; 2006 Feb; 97(3):414-9. PubMed ID: 15913994.
    Abstract:
    This study investigated four methods for the recovery of enteroviruses from sterilized raw wastewater, activated sludge, thickened sludge and treated wastewater, inoculated with Echovirus 11, Gregory prototype. The adsorption-elution method recommended by the US Environmental Protection Agency (EPA) was better for Echovirus 11 recovery than a sonication method, a modified EPA method and a membrane adsorption elution method since it resulted in the highest detection levels by cell culture and RT-PCR (Friedman's test, p<0.00041 and p=0.041, respectively).
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