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  • Title: [Effects of centrifuge training on mRNA expression in different rat tissues].
    Author: Li LP, Liu L, Yu LS, Luo CQ.
    Journal: Space Med Med Eng (Beijing); 2004 Jun; 17(3):184-8. PubMed ID: 15920844.
    Abstract:
    OBJECTIVE: To demonstrate the tissue specific expression of five rat genes related to centrifuge training and the relationship between expression change and duration of training. METHOD: mRNA was extracted from hearts, brains, kidneys, lungs and intestines of rats after different durations of training. Five genes obtained by SSH from centrifuge-trained rats were labeled by Dig-11-dUTP as probes. The expression levels were measured by thin-line hybridization. Optical density (OD) values were obtained by scanning and treated with statistics. RESULT: As compared with control group, the new gene CH157 expression decreased in hearts and brains of rats trained for 6 d (P<0.05), but returned to control levels after 12 d training; the new gene CH244 expression depressed in rat hearts, brains, and intestines after 6 d or 12 d training, but it was not statistically significant owing to large individual differences; gene expression of protein inhibitor of neuronal nitric oxide syntheses (PIN) increased gradually and significantly in rat hearts (P<0.01 after 12 d training) and kidneys (P<0.05 after 12 d training); Cyt b gene expression in rat hearts and brains increased increasingly (P<0.01 after 12 d training), however, it increased obviously (P<0.05) in kidneys, lungs and intestines of rats trained for 6 d, while it returned back a little in rats trained for 12 d; enlongation factor 1-alpha expression levels in intestines of rats trained for 6 d increased significantly (P<0.05), but it became no significant in rats trained for 12 d, while the expression in brains increased gradually (P<0.01). CONCLUSION: It suggested that the change in gene expression in several tissues of rats caused by centrifuge training contribute in enhancing +Gz tolerance.
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