These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Contamination-free continuous flow microfluidic polymerase chain reaction for quantitative and clinical applications.
    Author: Dorfman KD, Chabert M, Codarbox JH, Rousseau G, de Cremoux P, Viovy JL.
    Journal: Anal Chem; 2005 Jun 01; 77(11):3700-4. PubMed ID: 15924408.
    Abstract:
    We present a method for performing polymerase chain reaction (PCR) using isolated droplets flowing in an immiscible fluorinated solvent system. Thanks to an optimized control of interfacial properties, we could achieve in this capillary-based system reproducible amplification factors, without any detectable contamination between neighboring droplets. The system is readily amenable to further miniaturization and automation and serves as the first step toward a clinically viable, high-throughput, quantitative continuous flow PCR apparatus.
    [Abstract] [Full Text] [Related] [New Search]