These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Rapid determination of ultra trace amounts of residual protein in penicillin based on its enhancement effects of Rayleigh light scattering on trimethoxyphenlyfluorone (TM-PF)-Mo(VI) complex.
    Author: Qin W, Yan L, Hui Z, Yulong D, Bin D.
    Journal: J Pharm Biomed Anal; 2005 Jun 15; 38(2):232-8. PubMed ID: 15925213.
    Abstract:
    This is a report on the ultra trace amount determination of protein in penicillin based on the interaction with trimethoxyphenlyfluorone (TM-PF)-molybdenum (Mo)(VI) complex in the presence of Triton X-100 microemulsion by Rayleigh light scattering (RLS). At pH 2.70, the weak RLS of TM-PF-Mo(VI) complex was enhanced greatly by the addition of proteins. Based on this, the interaction of TM-PF-Mo(VI) complex with proteins was studied and a new quantitative determination method for proteins was developed. This method is extremely sensitive (the linear range of the calibration curve was 0-80 ng mL(-1) for bovine serum albumin (BSA). The detection limit was 0.47 ng mL(-1)), simple, steady and free from interference of coexisting substances. TM-PF-Mo(VI) complex as a spectral probe can be used to the determination of the residual protein in penicillin samples successfully.
    [Abstract] [Full Text] [Related] [New Search]