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  • Title: Genomic DNA amplification from a single bacterium.
    Author: Raghunathan A, Ferguson HR, Bornarth CJ, Song W, Driscoll M, Lasken RS.
    Journal: Appl Environ Microbiol; 2005 Jun; 71(6):3342-7. PubMed ID: 15933038.
    Abstract:
    Genomic DNA was amplified about 5 billion-fold from single, flow-sorted bacterial cells by the multiple displacement amplification (MDA) reaction, using phi 29 DNA polymerase. A 662-bp segment of the 16S rRNA gene could be accurately sequenced from the amplified DNA. MDA methods enable new strategies for studying non-culturable microorganisms.
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