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  • Title: The involvement of V(alpha)14 natural killer T cells in the pathogenesis of arthritis in murine models.
    Author: Chiba A, Kaieda S, Oki S, Yamamura T, Miyake S.
    Journal: Arthritis Rheum; 2005 Jun; 52(6):1941-8. PubMed ID: 15934073.
    Abstract:
    OBJECTIVE: To examine the physiologic role of natural killer T (NKT) cells bearing V(alpha)14 T cell receptor (TCR) in the pathogenesis of collagen-induced arthritis (CIA) and antibody-induced arthritis in mice. METHODS: NKT cells were stained with alpha-galactosylceramide-loaded CD1 dimer, and then assessed using flow cytometry. CIA was induced in mice by immunization on days 0 and 21 with type II collagen (CII) emulsified with an equal volume of Freund's complete adjuvant. Anti-CII antibodies were measured by enzyme-linked immunosorbent assay. For antibody-induced arthritis, mice were injected with anti-CII monoclonal antibodies (mAb) followed by lipopolysaccharide, or with serum from KRN TCR-transgenic mice crossed with nonobese diabetic mice (K/BxN). The severity of arthritis was monitored with a macroscopic scoring system. RESULTS: The number of NKT cells increased in the liver at the peak of the clinical course of CIA. Administration of anti-CD1 mAb inhibited development of CIA. The severity of CIA in NKT cell-deficient mice was reduced compared with that in wild-type mice. The IgG1:IgG2a ratio of anti-CII was elevated and production of interleukin-10 from draining lymph node cells was increased in NKT cell-deficient mice. NKT cell-deficient mice were significantly less susceptible to antibody-induced arthritis. CONCLUSION: NKT cells contribute to the pathogenesis of arthritis by enhancing autoantibody-mediated inflammation. NKT cells also contribute to the disease process in a deleterious way, due, at least in part, to the alteration of the Th1/Th2 balance in T cell response to CII.
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