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  • Title: [Identification and isolation of human dental pulp stem cells].
    Author: Yang XC, Fan MW.
    Journal: Zhonghua Kou Qiang Yi Xue Za Zhi; 2005 May; 40(3):244-7. PubMed ID: 15938892.
    Abstract:
    OBJECTIVE: To isolate and cultivate human dental pulp stem cells (DPSCs). METHODS: Pulp tissue was removed from healthy young human teeth extracted for orthodontic purposes. The pulp was digested by Type I collagenase and dispase. Then single-cell suspensions were obtained by filter and cultivated. The clones were identified by expression of STRO-1. Under the conditions of inducement, clones were identified by activity of alkaline phosphatase (ALP), formation of mineralized nodule and expression of dentin sialoprotein (DSP), and by Oil Red-O dyeing and expressing of PPARr2. RESULTS: The clones had positive expression of STRO-1. When stimulated to differentiation, these cells took on dramatically high activity of ALP, had the ability of mineralization and expressed DSP. These cells also had ability to trans-differentiate into adipocytes. CONCLUSION: There are stem cells in human dental pulp tissues, which can be isolated and cultivated.
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