These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: [Studies on the germination of Bacillus anthracoides spores induced by nutrient germinant].
    Author: Zhang LJ, Xiong HY, Zhang Y, Ma F, Li L.
    Journal: Zhonghua Liu Xing Bing Xue Za Zhi; 2005 Mar; 26(3):207-10. PubMed ID: 15941512.
    Abstract:
    OBJECTIVE: To explore the germination effects of Bacillus anthracoides spores germinant to nutrient germinant. METHODS: Heat factors and nutrient germinant were used to stimulate the Bacillus anthracoides spores and to germinate. Ultraviolet spectrophotometer was used to measured the A value of spore solution in the wavelength of 600 nm. Accrding to the A value, the germination rates in different condition. Transmission electron microscope was used to observe the ultrastructure changes of spores. RESULTS: The rate of germination effects were 68.0% under 6 mmol/L inosine at 37 degrees C, pH 7.9; 74.5% under 70 mmol/L L-alanine at 30 degrees C, pH 8.9; and 85.6% under 6 mmol/L inosine and 70 mmol/L L-alanine at 37 degrees C, pH 8.2. Under transmission electron microscope, the germinated spores' coat and cortex were brokendown and degraded with its core completely exposed. CONCLUSION: Under suitable environment, the nutrient germinant with inosine and L-alanine might be helpful for germinating the bacillus anthracoides spores.
    [Abstract] [Full Text] [Related] [New Search]