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  • Title: Laser-induced fluorescence detection schemes for the analysis of proteins and peptides using capillary electrophoresis.
    Author: Lacroix M, Poinsot V, Fournier C, Couderc F.
    Journal: Electrophoresis; 2005 Jun; 26(13):2608-21. PubMed ID: 15948219.
    Abstract:
    Over the past few years, a large number of studies have been prepared that describe the analysis of peptides and proteins using capillary electrophoresis (CE) and laser-induced fluorescence (LIF). These studies have focused on two general goals: (i) development of automatic, selective and quick separation and detection of mixtures of peptides or proteins; (ii) generation of new methods of quantitation for very low concentrations (nm and subnanomolar) of peptides. These two goals are attained with the use of covalent labelling reactions using a variety of dyes that can be readily excited by the radiation from a commonly available laser or via the use of noncovalent labelling (immunoassay using a labelled antibody or antigen or noncovalent dye interactions). In this review article, we summarize the works which were performed for protein and peptide analysis via CE-LIF.
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