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  • Title: [Determination of catechins and caffeine in tea and tea beverages by high-performance liquid chromatography].
    Author: Ling Y, Zhao YF, Li ZJ, Zhang G, Wu Y.
    Journal: Wei Sheng Yan Jiu; 2005 Mar; 34(2):187-90. PubMed ID: 15952660.
    Abstract:
    OBJECTIVE: A high-performance liquid chromatography method was developed for the determination of total tea catechin and caffeine. METHODS: The catechins needed to be determined are: (-)-catechin, (-)-epigallocatechin, (-)-epigallocatechin gallate, (-)-epicatechin, (-)-epicatechin gallate, (-)-gallocatechin, (-)-gallocatechin gallate, (-)-catechin gallate and caffeine. The catechins in the tea was extracted with water:ethanol (3:7) by sonication for 20 min. After the extraction solution was centrifuged, it was analyzed by HPLC. The column used was a CAPCELL PAK C18 MG (4.6mm I.D. x 150mm) and gradient elution at constant column temperature with 30 degrees C under UV detector with 210nm. Mobile phase A was 0.1% H3PO4 in water, mobile phase B was 0.1% H3PO4 in methanol with the flow rate 1ml/min. RESULTS: The results showed that the concentration of the catechins and caffeine and their peak areas achieved good linear relation, r > 0.999. The recoveries were between 61.7%-117.3%. RSD was below 10%. With this method, some tea samples were determined, the results were favorable. CONCLUSION: The method can be applied for determination of catechin and caffeine in tea and tea beverages.
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