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  • Title: Improved protein identification through the use of unstained gels.
    Author: Loiselle DR, Thelin WR, Parker CE, Dicheva NN, Kesner BA, Mocanu V, Wang F, Milgram SL, Warren MR, Borchers CH.
    Journal: J Proteome Res; 2005; 4(3):992-7. PubMed ID: 15952747.
    Abstract:
    In this work, a method for improved protein identification of low-abundance proteins using unstained gels, in combination with robotics and matrix-assisted laser desorption/ionization tandem mass spectrometry, has been developed and evaluated. Omitting the silver-staining process resulted in increased protein identification scores, an increase in the number of peptides observed in the MALDI mass spectrum, and improved quality of the tandem mass spectrometry data.
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