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  • Title: Fluorogenic peptide substrates containing benzoxazol-5-yl-alanine derivatives for kinetic assay of cysteine proteases.
    Author: Szabelski M, Rogiewicz M, Wiczk W.
    Journal: Anal Biochem; 2005 Jul 01; 342(1):20-7. PubMed ID: 15958176.
    Abstract:
    New peptide substrates containing benzoxazol-5-yl-alanine derivatives for kinetic assay of cysteine proteases have been synthesized and characterized. The substrates are peptides internally quenched by the intramolecular fluorescence resonance energy transfer. The results demonstrate that the kind of donor-acceptor pair (D-A) significantly affects the kinetic parameters of the enzymatic process. The three longest peptides, Box-Lys-Phe-Gly-Gly-Ala-Ala-Tyr(NO2) containing Box-alanine derivative as a donor and nitro-tyrosine as an acceptor, show two times greater affinity to papain than does the one peptide possessing Dabcyl-Edans as a D-A pair. Kinetic parameters for the best papain substrate, Lys-Box(benzfur)-Gly-Gly-Ala-Ala-Tyr(NO2), are Km = 6.85+/-0.59 microM, kcat = 19.51 s(-1), and kcat/Km = 2.85 microM(-1) s(-1). It was found that the peptides Box(benzfur)-Lys-Phe-Gly-Gly-Tyr(NO2) and Box(benzfur)-Phe-Gly-Gly-Tyr(NO2) were also hydrolyzed by cathepsin B with the highest speed of hydrolysis as a result of carboxypeptidase activity of this enzyme. Moreover, these substrates show high affinity and selectivity to this enzyme.
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