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  • Title: [Study on the differentiation of marrow stromal stem cells into neural cells in vitro].
    Author: Zhuo B, Jiang H, Qu P.
    Journal: Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi; 2005 May; 19(5):373-6. PubMed ID: 15960442.
    Abstract:
    OBJECTIVE: To investigate the neural markers' expression in the differentiation of marrow stromal stem cells (MSCs) into neural cells. METHODS: Rats MSCs were expanded as undifferentiated cells in vitro for 5 to 7 generations and cultured in a modified neuronal medium (MNM) after 24 hours of all-trans retinoid acid (ATRA) pretreatment. Immunocytochemistry was used to detect the expression of nestin, neuron-specific nuclear protein (NeuN), microtubule-associated protein 2 (MAP-2) and glial fibrillary acidic protein (GFAP) at different time points. RESULTS: After ATRA and MNM treatment, MSCs progressively assumed neuronal morphological characteristics. Nestin occurred first after 24 hours of ATRA treatment; then NeuN expressed after 2 hours of MNM treatment; the last one was MAP-2 and it was detected after 9 hours of MNM treatment. Other markers continuously expressed except that the expression of nestin peaked after 18 hours of MNM induction and remarkably decreased after 36 hours. CONCLUSION: ATRA and MNM could promote the differentiation of MSCs into neural cells and the expression of neural-specific markers was consistent with current knowledge regarding the time points of markers expression in the neuronal development which provides a good model in vitro for neuronal development research.
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