These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: PCR-generated artefact from 16S rRNA gene-specific primers.
    Author: Osborne CA, Galic M, Sangwan P, Janssen PH.
    Journal: FEMS Microbiol Lett; 2005 Jul 15; 248(2):183-7. PubMed ID: 15961258.
    Abstract:
    Artefacts consisting of concatenated oligonucleotide primer sequences were generated during sub-optimally performing polymerase chain reaction amplification of bacterial 16S rRNA genes using a commonly employed primer pair. These artefacts were observed during amplification for terminal restriction fragment length polymorphism analyses of complex microbial communities, and after amplification from DNA from a microbial culture. Similar repetitive motifs were found in gene sequences deposited in GenBank. The artefact can be avoided by using different primers for the amplification reaction.
    [Abstract] [Full Text] [Related] [New Search]