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  • Title: Strain improvement for tannase production from co-culture of Aspergillus foetidus and Rhizopus oryzae.
    Author: Purohit JS, Dutta JR, Nanda RK, Banerjee R.
    Journal: Bioresour Technol; 2006 Apr; 97(6):795-801. PubMed ID: 15963714.
    Abstract:
    Spores from the co-culture of Aspergillus foetidus and Rhizopus oryzae were subjected to UV, heat and NTG (3-nitro,5-methylguanidine) mutagenesis. A few colonies were screened from the selected media for tannase study. Amongst all, the best mutant isolated from the heat treatment (60 degrees C for 60 min) was SCPR 337. The maximum yield of gallic acid and tannase in case of mutant strain was 95.2% and 53.6 U/ml with an incubation period of 30 h as compared to wild strain where the incubation period was 48 h with an enzyme activity of 44.2 U/ml and gallic acid yield of 94%, respectively. The mutant was sensitive to tetracycline and was also an over-producer of protease and amylase.
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