These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Comparison of replicative senescence and stress-induced premature senescence combining differential display and low-density DNA arrays.
    Author: Pascal T, Debacq-Chainiaux F, Chrétien A, Bastin C, Dabée AF, Bertholet V, Remacle J, Toussaint O.
    Journal: FEBS Lett; 2005 Jul 04; 579(17):3651-9. PubMed ID: 15963989.
    Abstract:
    Human diploid fibroblasts (HDFs) exposed to subcytotoxic stress display many features of senescence. Using differential display RT-PCR, gene expression of HDFs in premature senescence induced by tert-butylhydroperoxide or ethanol and in replicative senescence was compared to gene expression of HDFs at early cumulative population doublings. Thirty genes of known function were identified from the 265 differentially displayed cDNA fragments. A customized low-density array allowed to confirm the relative level of the corresponding 30 transcripts. We found differential expression of genes coding for proteins implicated namely in growth arrest (PTEN, IGFBP-3, LRP-1 and CAV1), senescent morphogenesis (TGF-beta1 and LOXL2) and iron metabolism (TFR and FTL).
    [Abstract] [Full Text] [Related] [New Search]