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  • Title: [Isolation of thermophilic bacteria Thermus sp. YBJ-1 and cloning of amylase gene].
    Author: Xiong PJ, Wen JJ.
    Journal: Sheng Wu Gong Cheng Xue Bao; 2004 May; 20(3):434-6. PubMed ID: 15971619.
    Abstract:
    Thermophilic bacteria strain YBJ-1 was isolated from hot spring samples collected from Yangbajing, Tibet. The 16sr DNA sequence of YBJ-1 (1511bp in length) shares 98% identity with that of Thermus scotoductus strain ITI-252T. The full-length ORF of amylase gene of YBJ-1 (amyT) was amplified by PCR technique and cloned into T-vector. The complete sequence of amyT is 1767bp in length, coding for 588 amino acids. The deduced amino acids share 99% similarity with alpha-cyclodextrinse of Bacillus sterothermophilus, 96% with maltogenic amylse of Thermus. sp IM6501, and 81% with neopullulanase of Bacillus sterothermophlus.
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