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  • Title: Sequence analysis, cloning and over-expression of an endoxylanase from the alkaliphilic Bacillus halodurans.
    Author: Martínez MA, Delgado OD, Baigorí MD, Siñeriz F.
    Journal: Biotechnol Lett; 2005 Apr; 27(8):545-50. PubMed ID: 15973487.
    Abstract:
    The BhMIR32 xyn11A gene, encoding an extracellular endoxylanase of potential interest in bio-bleaching applications, was amplified from Bacillus halodurans MIR32 genomic DNA. The protein encoded is an endo-1,4-beta-xylanase belonging to family 11 of glycosyl hydrolases. Its nucleotide sequence was analysed and the mature peptide was subcloned into pET22b(+) expression vector. The enzyme was over-expressed in a high density Escherichia coli culture as a soluble and active protein, and purified in a single step by immobilised metal ion affinity chromatography with a specific activity of 3073 IU mg-1.
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