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  • Title: Purification and determination of plant hormones auxin and abscisic acid using solid phase extraction and two-dimensional high performance liquid chromatography.
    Author: Dobrev PI, Havlícek L, Vagner M, Malbeck J, Kamínek M.
    Journal: J Chromatogr A; 2005 May 20; 1075(1-2):159-66. PubMed ID: 15974129.
    Abstract:
    A method for separation and purification of plant hormones auxin and abscisic acid based on mixed mode reversed-phase anion-exchange solid phase extraction and two-dimensional HPLC was developed. Two-dimensional HPLC in "heart cutting" mode was very efficient in the purification of these two hormones. Its purification power is high enough to allow reliable on-line quantification of both hormones even with non-selective detectors.
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