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  • Title: Combining lectin microcolumns with high-resolution separation techniques for enrichment of glycoproteins and glycopeptides.
    Author: Madera M, Mechref Y, Novotny MV.
    Journal: Anal Chem; 2005 Jul 01; 77(13):4081-90. PubMed ID: 15987113.
    Abstract:
    Silica-based lectin microcolumns are described in this study together with the chemical procedures necessary for their preparation. The analytical merits of Canavalia ensiformis and Sambucus nigra lectins, [immobilized on activated macroporous silica], such as binding capacity, trapping reproducibility, and substrate selectivity, have been evaluated using model glycoproteins. The described microcolumns are applicable to high-pressure analytical schemes utilizing microvalving procedures, washing steps, and quantitative desorption for LC/MS analysis. The described analytical systems are amenable to the applications aiming at fractionation of complex glycopeptide mixtures and determination of the sites of glycosylation.
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