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  • Title: Template-directed DNA photoligation via alpha-5-cyanovinyldeoxyuridine.
    Author: Ogino M, Yoshimura Y, Nakazawa A, Saito I, Fujimoto K.
    Journal: Org Lett; 2005 Jul 07; 7(14):2853-6. PubMed ID: 15987153.
    Abstract:
    [reaction: see text] We describe an efficient template-directed photoligation of oligodeoxynucleotides (ODNs) using alpha-5-cyanovinyldeoxyuridine (alpha(C)U). An efficient photoligation was produced by photoirradiation of an ODN containing alpha(C)U at the 3' end with an ODN containing thymine at the 5' end in the presence of a template ODN. This photoligation method is a new and efficient way to synthesize branched ODNs.
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