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  • Title: Accelerating bacterial identification by infrared spectroscopy by employing microarray deposition of microorganisms.
    Author: Al-Khaldi SF, Mossoba MM, Ismail AA, Fry FS.
    Journal: Foodborne Pathog Dis; 2004; 1(3):172-7. PubMed ID: 15992277.
    Abstract:
    A microarray method for the deposition of bacteria onto an agar slide was developed to accelerate the formation of microcolonies. Representative microarrays each consisting of 40 micro-spots of five replicates of eight foodborne bacteria (Yersinia enterocolitica, Staphylococcus aureus, Salmonella typhimurium, Listeria monocytogenes, Enterobacter cloacae, Citrobacter freundii, Klebsiella pneumoniae, and Escherichia coli) were printed on a Brain Heart Infusion (BHI) agar slide using a contact micro-spotting robotic system. Within 3 h, sufficient bacterial cells were obtained to allow accurate identification of the microorganism by infrared spectroscopy. This approach allows a "complete-in-a-single-day" analysis of a large array of samples.
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