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  • Title: Genomic structure of the ME491/CD63 antigen gene and functional analysis of the 5'-flanking regulatory sequences.
    Author: Hotta H, Miyamoto H, Hara I, Takahashi N, Homma M.
    Journal: Biochem Biophys Res Commun; 1992 May 29; 185(1):436-42. PubMed ID: 1599482.
    Abstract:
    Genomic structure of the ME491/CD63 antigen gene and promoter activity of the 5'-flanking regulatory sequences were studied. The antigen gene consists of eight exons that span 4 kilobase-pairs. Primer extension analysis with RNA from cultured human cells identified three major transcription initiation sites. The 5'-flanking region of exon 1 has features characteristic to promoters of many house-keeping genes and growth-regulating genes. The region is highly GC rich and contains potential binding sites for transcription factors such as Sp1 and ETF, but not a TATA box. The 5'-flanking sequence exerted strong promoter activity when linked to a reporter gene. Deletion mutant analysis of the 5'-flanking sequence has strongly suggested that a potential binding site for AP-1 plays an important role in positively regulating the gene expression.
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