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  • Title: Multiple-mutation reaction: a method for simultaneous introduction of multiple mutations into the glpK gene of Mycoplasma pneumoniae.
    Author: Hames C, Halbedel S, Schilling O, Stülke J.
    Journal: Appl Environ Microbiol; 2005 Jul; 71(7):4097-100. PubMed ID: 16000825.
    Abstract:
    In Mycoplasma pneumoniae, the UGA opal codon specifies tryptophan rather than a translation stop site. This often makes it difficult to express Mycoplasma proteins in E. coli isolates. In this work, we developed a strategy for the one-step introduction of several mutations. This method, the multiple-mutation reaction, is used to simultaneously replace nine opal codons in the M. pneumoniae glpK gene.
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