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  • Title: [Experimental study on the effects of interferon-gamma gene on Tenon's capsule fibroblasts in vitro].
    Author: Lan YQ, Ge J, Lin MK, Zheng JL, Wei J, Liu HQ, Li YY.
    Journal: Zhonghua Yan Ke Za Zhi; 2005 Jun; 41(6):527-32. PubMed ID: 16008914.
    Abstract:
    OBJECTIVE: To investigate the effects of interferon-gamma (IFN-gamma) gene on Tenon's capsule fibroblasts in vitro. METHODS: Using lipofectAMINE, IFN-gamma gene was transferred to human Tenon's capsule fibroblasts with plasmid pcDNA3IFN-gamma in vitro. Cells treated with plasmid pcDNA3 without IFN-gamma gene were used as the controls. The transfected fibroblasts were screened by G418. The expression of IFN-gamma was determined by RT-PCR, immunohistochemistry and flow cytometry assay. The effects of IFN-gamma on the proliferation of Tenon's capsule fibroblasts were evaluated by flow cytometry and MTT. RESULTS: The Tenon's capsule fibroblasts transferred the IFN-gamma gene could express the IFN-gamma RNA and protein transiently. The proliferation of the fibroblasts transferred the IFN-gamma gene was inhibited. CONCLUSION: The proliferation of the Tenon's capsule fibroblasts in vitro can be inhibited by transferred IFN-gamma gene.
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