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  • Title: Role of mannose receptor in oligochitosan-mediated stimulation of macrophage function.
    Author: Han Y, Zhao L, Yu Z, Feng J, Yu Q.
    Journal: Int Immunopharmacol; 2005 Sep; 5(10):1533-42. PubMed ID: 16023605.
    Abstract:
    In this paper, we aimed to study the role of mannose receptor (MR) in oligochitosan induced RAW264.7 (a murine macrophage cell line) activation. Oligochitosan, which has 3-10 saccharide (N-acetyl-glucosamine or glucosamine) residues, was prepared by enzymatic hydrolysis of chitosan using cellulase. Fluorophore 2-aminoacridone was conjugated to oligochitosan to observe cellular events of oligochitosan-RAW264.7 interaction under Confocal Laser Microscopy. RT-PCR was performed to assess the level of tumor necrosis factor-alpha (TNF-alpha) secretion. Free cytosolic Ca(2+) was measured using the fluorescent Ca(2+) indicator Fluo3/AM. We found that MR was the major receptor responsible for oligochitosan uptake. MR was also engaged in oligochitosan induced TNF-alpha enhancement and [Ca(2+)](in) flux and may serve as a signaling receptor in oligochitosan-induced activation of macrophage function.
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