These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: [Clone expression and purification of influenza A virus nucleoprotein gene].
    Author: Fang SS, Cheng XW, Liu T, Liu JJ, Zhao SJ, Zhou L.
    Journal: Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi; 2005 Jun; 19(2):165-8. PubMed ID: 16027788.
    Abstract:
    OBJECTIVE: To clone the influenza A virus NP gene into expression vector and to purify the target protein, which was used to study the preparation of monoclonal antibody. METHODS: The RNA of influenza A virus was extracted and primers were designed according the NP gene sequence, then the NP gene of influenza A virus was expressed in E.coli DH5alpha and the NP protein was purified by affinity chromatography. RESULTS: The recombinant expression vector-pGEX-4T-2-NP was successfully constructed and relatively pure target protein was obtained. CONCLUSION: Through reasonably controlling the fermentation time, growth temperature and induction concentration, satisfactory soluble target product was obtained.
    [Abstract] [Full Text] [Related] [New Search]