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  • Title: Quenched fluorescent substrate-based peptidase assays.
    Author: Lew RA, Tochon-Danguy N, Hamilton CA, Stewart KM, Aguilar MI, Smith AI.
    Journal: Methods Mol Biol; 2005; 298():143-50. PubMed ID: 16044545.
    Abstract:
    The use of specific quenched fluorescent substrates (QFS) provides a rapid and sensitive method to measure peptidase activity, and is readily adaptable to high-throughput screening of potential peptidase inhibitors. In this chapter, we discuss general considerations for the development of QFS assays, and describe in detail an assay protocol for the mammalian metallopeptidase, endothelin-converting enzyme.
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