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  • Title: An alternative protocol for DNA extraction from formalin fixed and paraffin wax embedded tissue.
    Author: Coura R, Prolla JC, Meurer L, Ashton-Prolla P.
    Journal: J Clin Pathol; 2005 Aug; 58(8):894-5. PubMed ID: 16049299.
    Abstract:
    BACKGROUND: DNA extraction from paraffin wax embedded tissue requires special protocols, and most described methods report an amplification success rate of 60-80%. AIMS: To propose a simple and inexpensive protocol consisting of xylene/ethanol dewaxing, followed by a kit based extraction. METHOD: Xylene/ethanol dewaxing was followed by a long rehydration step and a kit based DNA extraction step. RESULTS: This method produced a 100% amplification success rate for fragments of 121 to 227 bp for tamponated formalin fixed paraffin wax embedded tissue. CONCLUSION: This cost effective and non-laborious protocol can successfully extract DNA from tamponated formalin fixed paraffin wax embedded tissue and should facilitate the molecular analysis of a large number of archival specimens in retrospective studies.
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