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  • Title: Use of chlorite to improve HPLC detection of pyridoxal 5'-phosphate.
    Author: Ericson KL, Mahuren JD, Zubovic YM, Coburn SP.
    Journal: J Chromatogr B Analyt Technol Biomed Life Sci; 2005 Sep 05; 823(2):218-20. PubMed ID: 16054880.
    Abstract:
    The sensitivity of fluorescent detection of the biologically active form of Vitamin B-6, pyridoxal 5'-phosphate (PLP), in biological samples has been improved approximately four-fold by adopting chlorite as a post-column derivatization reagent (instead of bisulfite) in high-performance liquid chromatography (HPLC) separation. Chlorite oxidizes PLP to the more fluorescent 4-pyridoxic acid 5'-phosphate, and avoids the toxicity and heating of the cyanide procedure. Detection of another major metabolite, 4-pyridoxic acid (4-PA), is not effected. Detection of pyridoxal (PL) is slightly lowered due to eluting at a lower pH.
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