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  • Title: Is the Fab-fragment from monoclonal rheumatoid IgM flexible? A spin label dynamics study.
    Author: Timofeev VP, Tkachev YV, Novikov VV, Alyoshkin VA, Lapuk VA.
    Journal: J Biomol Struct Dyn; 2005 Oct; 23(2):175-81. PubMed ID: 16060691.
    Abstract:
    A comparative study of the Fab- and Fab-RF-fragments in the molecules of monoclonal IgM and IgM-RF, respectively, was performed by the spin label method. The spin label, 2,2,6,6-tetramethyl-4-dichloro-triazinylaminopiperidine-1-oxyl, was introduced into the peptide part of the protein. On the basis of the data on the temperature-viscosity dependences of the EPR spectral parameters of the resultant spin-labeled proteins, the rotational correlation time tau of the spin carrier was determined. It turned out that the reduced to normal conditions tau values for the molecules of the Fab- and Fab-RF-fragments were 21+/-2 and 11+/-1 ns, respectively. Analysis of the resultant data provides sufficient grounds for assuming that such a sharp decrease in the tau value for the molecule of the Fab-RF fragment is due to local flexibility of its globular structure, which, in turn, can determine the specific features of the IgM-RF functioning as an autoantibody.
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