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  • Title: Determination of nitrofuran metabolites in poultry muscle and eggs by liquid chromatography-tandem mass spectrometry.
    Author: Finzi JK, Donato JL, Sucupira M, De Nucci G.
    Journal: J Chromatogr B Analyt Technol Biomed Life Sci; 2005 Sep 25; 824(1-2):30-5. PubMed ID: 16061430.
    Abstract:
    The use of nitrofurans in food-producing animals has been banned in EU. Detection of the protein-bound nitrofuran metabolites is the best approach to evaluate their utilization. A fast, sensitive and reliable LC-MS-MS method is presented to analyze simultaneously the metabolites of four commonly used nitrofuran drugs, furazolidone, furaltadone, nitrofurazone and nitrofurantoin. The sample clean up was performed by a single liquid-liquid extraction step, after a hydrolysis and derivatisation process. Separation of the molecules was performed by liquid chromatography in a C18 column (100 mmx2.1 mm, 4 microm) at room temperature. The quantitative and confirmatory determination of these metabolites was performed by multiple reactions monitoring (MRM). Limits of quantification of 0.5 ngg(-1) were achieved and the total analysis was accomplished in 5 min. This protocol has been applied to identify contaminated samples of poultry muscle and egg products.
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