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  • Title: Detection of arsenical drug resistance in Trypanosoma brucei with a simple fluorescence test.
    Author: Stewart ML, Krishna S, Burchmore RJ, Brun R, de Koning HP, Boykin DW, Tidwell RR, Hall JE, Barrett MP.
    Journal: Lancet; ; 366(9484):486-7. PubMed ID: 16084257.
    Abstract:
    The resurgence of human African trypanosomiasis (HAT), coupled with an increased incidence of drug resistance, is of concern. We report a quick, simple, and sensitive test for identification of parasites resistant to melarsoprol, the main drug used to treat late stage HAT. Resistant parasites are defective in a plasma membrane transporter responsible for drug uptake. The same transporter carries the fluorescent diamidine DB99 (2,5-bis-(4-amidinophenyl)-3,4-dimethylfuran) into trypanosomes. The two DNA-containing structures in the trypanosome--the nucleus and the kinetoplast--begin to fluoresce within 1 min of introduction of DB99, unless drug resistant.
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