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  • Title: HPLC-DAD for analysis of different classes of drugs in plasma.
    Author: Alabdalla MA.
    Journal: J Clin Forensic Med; 2005 Dec; 12(6):310-5. PubMed ID: 16084748.
    Abstract:
    A solid-phase extraction technique and HPLC-DAD method for drug screening in plasma were developed. This analytical method extracts and tests a number of drugs of different classes. The method includes; an acidic and basic Solid Phase Extraction (SPE) of plasma with C18 cartridges, a gradient elution of a modified cyano column with acidic buffer/acetonitrile eluent and a photodiode array ultraviolet (UV) detection. The drug screening procedure applied uses retention index and UV spectral data for the identification of compounds, may be appropriate in particular laboratory settings.
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