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  • Title: LuxS/autoinducer-2 quorum sensing molecule regulates transcriptional virulence gene expression in Clostridium difficile.
    Author: Lee AS, Song KP.
    Journal: Biochem Biophys Res Commun; 2005 Sep 30; 335(3):659-66. PubMed ID: 16098481.
    Abstract:
    Toxigenic Clostridium difficile CCUG19126 produces the autoinducer-2 (AI-2) quorum sensing molecule that induces bioluminescence in Vibrio harveyi BB170 reporter strain. AI-2-containing cell-free supernatants from mid-log phase C. difficile and Escherichia coli DH5alpha expressing recombinant luxS(cd) upregulated the transcript levels of tcdA (7-10-fold), tcdB (4-6-fold), and tcdE (2-3-fold) in early-log C. difficile. In contrast, no induction occurred when cells were exposed to sterile medium or to cell-free supernatant from E. coli DH5alpha. AI-2 did not significantly increase the level of toxin A in early-log C. difficile. These results suggest that LuxS(cd)-dependent signaling regulates virulence gene expression at the transcriptional level in C. difficile.
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