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  • Title: [Studies on the extraction and purification of total saponins from Parched Semen Ziziphi Spinosae].
    Author: Wu Y, Ding A, Bao B.
    Journal: Zhong Yao Cai; 2005 Mar; 28(3):219-23. PubMed ID: 16107032.
    Abstract:
    OBJECTIVE: To study the extraction and purification process of the total saponin from Parched Semen Ziziphi Spinosae with ethanol and macroporous resin. METHOD: The total saponins were extracted with ethanol and purified with macroporous resin by orthogonal design, taking content and purity of jujuboside A as guideline. RESULTS: The optimum extraction condition was adding 6 times amount of 80% ethanol and refluxing 3 times, for 30 minutes each time. The purification process with macroporous resin HPD-100 was using 0.5% NaOH (150ml), 30% ethanol (150ml) to wash out impurity, and 70% ethanol 50 ml to wash out saponin. The purity of jujuboside A was up to 17.9% and the eluted ratio 72.8%.
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