These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: [Tissue culture and plantlet regeneration from embryo of Saussurea involucrate].
    Author: Wu LQ, Guo SX, Xiao PG.
    Journal: Zhongguo Zhong Yao Za Zhi; 2005 Jun; 30(11):814-6. PubMed ID: 16110858.
    Abstract:
    OBJECTIVE: To establish a protocol of rapid clonal propagation of Saussurea involucrate by using embryo as explants. METHOD: MS medium was used as basal medium and BA and NAA were supplemented to find out the optimal hormone combinations for adventitious buds initiation, adventitious bud multiplication and rooting. RESULT: All embryo explants started to grow adventitious buds within 45 days when they were cultured on the media supplemented with 0.02-0.1 mg x L(-1) NAA and 2 mg x L(-1) BA; The adventitious buds multiplicated within 30 days when they were transferred to the media containing 0.1-0.2 mg x L(-1) NAA and 2-3 mg x L(-1) BA; 92% of the adventitious buds rooted well after they were planted on the MS medium containing macroelements at half strength and 0.4 mg x L(-1) NAA for 25 days. The regenerated plantlets grew well after they were transplanted and the survival rate was up to 70%. CONCLUSION: Plantlets of S. invducrate regenerated high frequence through adventitious bud.
    [Abstract] [Full Text] [Related] [New Search]