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  • Title: Comparison of measured oxyhemoglobin saturation and oxygen content with analyzer-calculated values and hand-calculated values obtained in unsedated healthy dogs.
    Author: Scott NE, Haskins SC, Aldrich J, Rezende M, Gallagher RM, Henderson MM.
    Journal: Am J Vet Res; 2005 Jul; 66(7):1273-7. PubMed ID: 16111169.
    Abstract:
    OBJECTIVE: To compare direct measurements of canine oxyhemoglobin (HbO2) saturation and blood oxygen content (ContO2) in healthy dogs with analyzer-calculated values derived by use of a human HbO2 relationship and with hand-calculated values derived by use of a canine HbO2 relationship. ANIMALS: 17 healthy dogs. PROCEDURE: 3-mL samples of heparinized arterial and jugular venous blood were collected from each dog. The pH, Pco2, Po2, hemoglobin, HbO2, carboxyhemoglobin, methemoglobin, and ContO2 were measured; HbO2 and ContO2 were calculated automatically by analyzers and also hand-calculated. Blood gas analyzer-calculated and hand-calculated HbO2 values were compared with co-oximeter-measured HbO2 values. Analyzer-calculated and hand-calculated ContO2 values were compared with oxygen content analyzer-measured values. RESULTS: Hand-calculated HbO2 values for arterial and jugular venous samples were slightly but significantly lower than those calculated by a blood gas analyzer or obtained from a co-oximeter. Hand-calculated and analyzer-calculated arterial and venous ContO2 were similar to measured values. CONCLUSIONS AND CLINICAL RELEVANCE: Although certain HbO2 and ContO2 values generated by use of the different methods were significantly different, these differences are unlikely to be clinically important in healthy dogs.
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