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  • Title: [Effects of bone marrow fibroblasts conditioned media on the expansion of megakaryocytes in vitro].
    Author: Zhou XY, Huang YH, Wang QR, Lu GX, Tan MQ.
    Journal: Zhong Nan Da Xue Xue Bao Yi Xue Ban; 2004 Dec; 29(6):658-61. PubMed ID: 16114551.
    Abstract:
    OBJECTIVE: To investigate the effect of bone marrow fibroblasts conditioned media on the expansion of megakaryocytes in vitro. METHODS: Serum-free bone marrow fibroblasts conditioned medium (BMF-CM) was prepared and collected. The effect of BMF-CM or BMF-CM combined with IL-11 on the expansion of mature megakaryocytes and the effect of BMF-CM on the expansion of megakaryocytic progenitors were observed. RT-PCR was applied to determine the expression of NF-E2 mRNA in human megakaryoblastic leukemia cell line--Meg-0l. RESULTS: BMF-CM promoted the expansion of mature megakaryocytes. The effect of BMF-CM combined with IL-11 on the expansion of mature megakaryocytes was further enhanced. BMF-CM promoted the expansion of megakaryocytic progenitors. NF-E2 mRNA expression in megakaryocytic cell lines Meg-0l was enhanced when cultures were exposed to 30% BMF-CM for 4 hours, compared with control cultures exposed to IMDM for 4 hours. CONCLUSION: BMF-CM can promote the expansion of megakaryocytes in vitro.
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