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  • Title: Identification of Casuarina-Frankia strains by use of polymerase chain reaction (PCR) with arbitrary primers.
    Author: Sellstedt A, Wullings B, Nyström U, Gustafsson P.
    Journal: FEMS Microbiol Lett; 1992 May 15; 72(1):1-5. PubMed ID: 1612411.
    Abstract:
    Free-living N2-fixing Frankia strains isolated from Casuarina sp. were investigated for genomic polymorphism. We used six 10-mer oligonucleotides as single arbitrary primers (AP) for the polymerase chain reaction (PCR) in order to amplify random DNA fragments in the genome of free-living Frankia strains. Agarose-gels of the amplified genomic DNA revealed that two of the six arbitrary primers showed polymorphism in the eight different Frankia genomes. Analysis of the AP-PCR products showed 9 polymorphic bands ranging from 4.1-0.60 kb. We conclude that single arbitrary primers can be used to amplify genomic DNA, and that polymorphism can be detected between the amplification products of the different Frankia genomes.
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