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  • Title: [Effects of celecoxib on the proliferation and apoptosis of human colorectal cancer cell line HT-29].
    Author: Peng J, Zhang GY, Xiao ZQ.
    Journal: Zhong Nan Da Xue Xue Bao Yi Xue Ban; 2004 Jun; 29(3):261-5. PubMed ID: 16136956.
    Abstract:
    OBJECTIVE: To determine the effect of Celecoxib on the proliferation and apoptosis of human colorectal cancer cell line HT-29 and the probable mechanism involved. METHODS: Using MTT assay, flow cytometry, Acridine orange and Ethidium bromide staining, and Western blotting analysis, the effects of celecoxib on the proliferation and apoptosis of HT-29 cells as well as its related mechanism were studied. RESULTS: The growth of HT-29 cell was inhibited by celecoxib in a dose- and time-dependent manner. Sub-G1 peak was detected by FCM, and apoptotic rate was between (7.31 +/- 2.37) % and (48.30 +/- 2.86) %. The cell ratio of G0/G1 phase increased,whereas the cell ratio of S and G2/M phases decreased in a dose-dependent manner after the treatment. The HT-29 cell line exhibited some morphologic features of apoptosis, including cell shrinkage, nuclear condensation, DNA fragmentation, and formation of apoptosis bodies, and the apoptotic rate was in a dose- and time-dependent manner. Celecoxib down-regulated the expression of CDK2, CDK4 and up-regulated the expression of P2 WAF1/CIP1 CONCLUSION: The effect of celecoxib inhibiting cell HT-29 proliferation and inducing cell apoptosis may relate to its blocking cell cycle progress.
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